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Qom Univ Med Sci J 2021, 15(2): 110-119 Back to browse issues page
Assessment of the Effects of Cyclooxygenase Inhibitors on the Immune Status Following Surgery in Adult Male Rats
Mohammad Abyari1 , Samad Alimohammadi * 2, Mehrdad Pooyanmehr1 , Ali Ghashghaii1 , Ali Maleki3
1- Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.
2- Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran. , s.alimohammadi@razi.ac.ir
3- Kermanshah University of Medical Sciences
Abstract:   (916 Views)
Background and Objectives: Surgery via different mechanisms causes immunosuppression in the postoperative
period. The aim of the present study was to investigate the effects of preoperative administration
of cyclooxygenase inhibitors on blood levels of Interleukin-2 (IL-2), Interferon-γ (IFN-γ), Tumor
Necrosis Factor-α (TNF-α), white blood cells (WBCs) and lymphocytes.
Methods: Seventy-five male Wistar rats were divided into five groups (n=15 for each group). Group 1 was
gavaged with normal saline for 3 consecutive days without surgery. Group 2 was gavaged with normal
saline for 3 consecutive days. Groups 3 to 5 were gavaged with indomethacin (4 mg/kg) and celecoxib
(7.5 and 15 mg/kg), respectively, for 3 consecutive days. In Groups 2 to 5, one hour after drugs administration
on the third day, laparotomy was used as a surgery pattern. Blood samples were collected 24
hours after surgery and levels IL-2, IFN-γ, TNF-α, WBCs and lymphocytes were determined. Data were
analyzed using one-way ANOVA test.
Results: Based on the results, a decrease in blood levels of IL-2, IFN-γ and lymphocytes along with an
increase in TNF-α and WBCs were observed 24 hours after surgery compared to control rats (P<0.05).
Indomethacin (4 mg/kg) and celecoxib (15 mg/kg) improved the decreased level of IL-2 (P<0.05). There
was no significant difference in IFN-γ and lymphocytes between surgery group and the groups treated
with indomethacin or celecoxib (P>0.05). Also, only celecoxib (15 mg/kg) could decrease the elevated
levels TNF-α and WBCs (P<0.05).
Conclusion: Perioperative use of cyclooxygenase inhibitors can improve immune system function.
Keywords: Immune system, Cyclooxygenase inhibitors, Surgery, Rat
Full-Text [PDF 4773 kb]   (497 Downloads)    
Type of Study: Original Article | Subject: فیزیولوژی
Received: 2021/05/2 | Accepted: 2021/05/16 | Published: 2021/04/19
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Abyari M, Alimohammadi S, Pooyanmehr M, Ghashghaii A, Maleki A. Assessment of the Effects of Cyclooxygenase Inhibitors on the Immune Status Following Surgery in Adult Male Rats. Qom Univ Med Sci J 2021; 15 (2) :110-119
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