Volume 15, Issue 10 (January 2022)
Qom Univ Med Sci J 2022, 15(10): 718-725 |
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Hoseinian M, Jalali Tehrani H, Ashabi G, Khalifeh S, Kheradmand A. The Effect of Maternal Morphine Addiction on Neural Plasticity of Fetal Brain in Wistar Rats. Qom Univ Med Sci J 2022; 15 (10) :718-725
URL: http://journal.muq.ac.ir/article-1-3333-en.html
URL: http://journal.muq.ac.ir/article-1-3333-en.html
Monir Hoseinian1 
, Hora Jalali Tehrani1 , Ghorbangol Ashabi2 , Solmaz Khalifeh3 , Afshin Kheradmand * 4
, Hora Jalali Tehrani1 , Ghorbangol Ashabi2 , Solmaz Khalifeh3 , Afshin Kheradmand * 4
1- Department of Developmental Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
2- Department of Physiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
3- Cognitive and Neuroscience Research Center (CNRC), Tehran Medical Sciences, Amir-Almomenin Hospital, Islamic Azad University, Tehran, Iran.
4- 4. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Iran University of Medical Sciences, Tehran, Iran.
2- Department of Physiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
3- Cognitive and Neuroscience Research Center (CNRC), Tehran Medical Sciences, Amir-Almomenin Hospital, Islamic Azad University, Tehran, Iran.
4- 4. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Iran University of Medical Sciences, Tehran, Iran.
Abstract: (1370 Views)
Background and Objectives: Prenatal exposure to morphine has long-lasting effects on synaptic plasticity. Brain-derived neurotrophic factor (BDNF) plays an important role in neuronal survival and growth, serves as a neurotransmitter modulator, and participates in neuronal plasticity, which is essential for cognition, learning and memory. Extracellular signal-regulated kinase (ERK) is one of the important downstream proteins of BDNF- tropomycin receptor kinase B (TrkB) signaling. Exact effect of opioids on ERK expression in the neuronal cells is still unclear. The aim of this study was to evaluate the effect of prenatal morphine exposure on BDNF and ERK protein levels in the rat fetal brain.
Methods: Pregnant rats (n=8) received increasing daily doses of morphine (0.1-0.4 mg/kg) in their drinking water. The brains of fetuses from both morphine-addicted and controls mothers were isolated at day 19 of gestation, and the levels of BDNF and p-ERK were measured using Western-blotting assay. The data presented as Mean±SEM and statistical analysis was performed by Unpaired T-test to compare between two groups.
Results: Our data revealed that the level of BDNF and p-ERK significantly decreased in the fetus’s brain of morphine addicted mothers comparing to control group.
Conclusion: Chronic exposure to morphine prenatally, could affect and diminish structural plasticity in the developing rat brain. It is probable that morphine exert this effect by reduction of BDNF level that consequently attenuate ERK phosphorylation.
Methods: Pregnant rats (n=8) received increasing daily doses of morphine (0.1-0.4 mg/kg) in their drinking water. The brains of fetuses from both morphine-addicted and controls mothers were isolated at day 19 of gestation, and the levels of BDNF and p-ERK were measured using Western-blotting assay. The data presented as Mean±SEM and statistical analysis was performed by Unpaired T-test to compare between two groups.
Results: Our data revealed that the level of BDNF and p-ERK significantly decreased in the fetus’s brain of morphine addicted mothers comparing to control group.
Conclusion: Chronic exposure to morphine prenatally, could affect and diminish structural plasticity in the developing rat brain. It is probable that morphine exert this effect by reduction of BDNF level that consequently attenuate ERK phosphorylation.
Type of Study: Original Article |
Subject:
فیزیولوژی
Received: 2021/12/7 | Accepted: 2022/02/5 | Published: 2022/01/30
Received: 2021/12/7 | Accepted: 2022/02/5 | Published: 2022/01/30
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