Volume 15, Issue 11 (February 2022)                   Qom Univ Med Sci J 2022, 15(11): 744-755 | Back to browse issues page

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Mirazi N, Shahabi Baher A, Izadi Z, Nourian A, Safari S. Study of Protective Effects of Calendula Officinalis L. Hydrethanolic Extract on Blood Parameters in Wistar Rats Treated With Cyclophosphamide. Qom Univ Med Sci J 2022; 15 (11) :744-755
URL: http://journal.muq.ac.ir/article-1-3343-en.html
1- Department of Biology, Faculty of Basic Sciences, Bu Ali Sina University, Hamedan, Iran. , mirazi205@gmail.com
2- Department of Biology, Faculty of Basic Sciences, Bu Ali Sina University, Hamedan, Iran.
3- Department of Horticulture sciences and Engineering, Nahavand Higher Education Complex, Bu-Ali Sina University, Hamedan, Iran
4- Department of Pathobiology, Faculty of Veterinary Medicine, Bu Ali Sina University, Hamedan, Iran
Abstract:   (1488 Views)
Background and Objectives: Some of drugs which used in chemotherapy have destructive effects on hemopoetic tissue. Cyclophosphamid is a drug for malignant cancers treatment with subversion effects on blood hemopoetic bone marrow. Medicinal plants with antioxidant effects have anti-inflammatory and protective effects on bone marrow tissue. The aim of this study is to investigate of protective effect of Calendula officinalis L. hydrethanolic extract (CHE) on boold parameters in Wistar male rats induced with cyclophosphamide.
Methods: In this experimental study 35 male Wistar rats (200-220 g) were divided in 5 groups(n=7): control (0.5 ml salin normal), Cyclophosphamide (15 mg/kg), positive control 600 mg/kg, CHE), treatment 1 (Cyclophosphamide 15 mg/kg‌+300 mg/kg, CHE) and treatment 2 (Cyclophosphamide 15mg/kg +600 mg/kg, CHE). All injections were done interaperituneally and the experiments were done for 15 days. At the end of expriments all animals were anesthetized (ketamine hydrochlorids, 50mg/kg+xylasine hydrochloride 10 mg/kg) and blood samples and sternum bone tissue (fixed in 10% formaldehyde) were collected. All data were evaluated statistically by ANOVA and Tukey’s method.
Results: The results of this study were showed that cyclophosphamide has reduce effects on blood parameters (RBC, WBC, PL, Hb, Hct, MCV,MCH, MCHC) compare with control group significantly. CHE can protect and inhibite of cyclophosphamide damage effects on blood hemopoetic tissue.
Conclusion: The use of CHE is able to protect of blood hemopoetic tissue in bone marrow and prohihite of cyclophosphamide effects.
Full-Text [PDF 2188 kb]   (355 Downloads)    
Type of Study: Original Article | Subject: فیزیولوژی
Received: 2021/12/18 | Accepted: 2022/01/23 | Published: 2022/01/30

1. Zhao CY, Cheng R, Yang Z, Tian ZM. Nanotechnology for cancer therapy based on chemotherapy. Molecules. 2018; 23(4):826. [PMID] [PMCID] [DOI:10.3390/molecules23040826]
2. Duggina P, Kalla CM, Varikasuvu SR, Bukke S, Tartte V. Protective effect of centella triterpene saponinsagainst cyclophosphamide-induced immune and hepatic system dysfunction in rats: Its possible mechanisms of action. J Physiol Biochem. 2015; 71(3):435-54. [DOI:10.1007/s13105-015-0423-y] [PMID] [DOI:10.1007/s13105-015-0423-y]
3. Bokser L, Szende B, Schally AV. Protective effect of D-Type 6-lutenizing hormone-realising hormone micro capsule against cyclophosphamide-induced gonadotoxicity in feamale rats. Br J Cancer. 1990; 61(6):861-5. [DOI:10.1038/bjc.1990.192] [PMID] [PMCID] [DOI:10.1038/bjc.1990.192]
4. Taherkhani B, Mirazi N. [Study of vitis vinifera L. seed's hydroethanolic extract on blood parameters in male rats induced with cyclophosphamide (Persian)]. Razi J Med Sci. 2019; 26(7):24-32. [Link]
5. Barnes H, Holland AE, Westall GP, Goh NS, Glaspole IN. Cyclophosphamide for connective tissue disease-associated interstitial lung disease. Cochrane Database Syst Rev. 2018; 1(1):CD010908. [DOI:10.1002/14651858.CD010908.pub2] [PMID] [DOI:10.1002/14651858.CD010908.pub2]
6. Kassa Z, Asfaw Z, Demissew S. An ethnobotanical study of medicinal plants in sheka zone of southern nations nationalities and peoples regional state, Ethiopia. J Ethnobiol Ethnomed. 2020; 16(1):7. [DOI:10.1186/s13002-020-0358-4] [PMID] [PMCID] [DOI:10.1186/s13002-020-0358-4]
7. Wangchuk P, Yeshi K, Jamphel K. Pharmacological, ethnopharmacological and botanical evaluation of subtropical medicinal plants of Lower Kheng region in Bhutan. Integr Med Res. 2017; 6(4):372-87. [DOI:10.1016/j.imr.2017.08.002] [PMID] [PMCID] [DOI:10.1016/j.imr.2017.08.002]
8. Andersen FA, Bergfeld WF, Belsito DV, Hill RA, Klaassen CD, Liebler DC, et al. Final report of the cosmetic ingredient review expert panel amended safety assessment of Calendula officinalis-derived cosmetic ingredients.Int J Toxicol. 2010; 29(6 Suppl):221S-43. [DOI:10.1177/1091581810384883] [PMID] [DOI:10.1177/1091581810384883]
9. Nicolaus C, Junghanns S, Hartmann A, Murillo R, Ganzera M, Merfort I. In vitro studies to evaluate the wound healing properties of Calendula officinalis extracts. J Ethnopharmacol. 2017; 196:94-103.[DOI:10.1016/j.jep.2016.12.006] [PMID] [DOI:10.1016/j.jep.2016.12.006]
10. Efstratiou E, Hussain AI, Nigam PS, Moore JE, Ayub MA, Rao JR. Antimicrobial activity of Calendula officinalis petal extracts against fungi, as well as gram-negative and gram-positive clinical pathogens. Complement Ther Clin Pract. 2012; 18(3):173-6.[DOI:10.1016/j.ctcp.2012.02.003] [PMID] [DOI:10.1016/j.ctcp.2012.02.003]
11. Cruceriu D, Balacescu O, Rakosy E. Calendula officinalis: Potential roles in cancer treatment and palliative care. Integr Cancer Ther. 2018; 17(4):1068-78.[DOI:10.1177/1534735418803766] [PMID] [PMCID] [DOI:10.1177/1534735418803766]
12. Tanideh N, Jamshidzadeh A, Sepehrimanesh M, Hosseinzadeh M, Koohi-Hosseinabadi O, Najibi A, et al. Healing acceleration of acetic acid-induced colitis by marigold (Calendula officinalis) in male rats. Saudi J Gastroenterol. 2016; 22(1):50-6. [PMID] [PMCID] [DOI:10.4103/1319-3767.173759]
13. Robinson D, Schulz G, Langley R, Donze K, Winchester K, Rodgers C. Evidence-based practice recommendations for hydration in children and adolescents with cancer receiving intravenous cyclophosphamide. J Pediatr Oncol Nurs. 2014; 31(4):191-9. [PMID] [PMCID] [DOI:10.1177/1043454214532024]
14. Huyan XH, Lin YP, Gao T, Chen RY, Fan YM. Immunosuppressive effect of cyclophosphamide on white blood cells and lymphocyte subpopulations from peripheral blood of Balb/c mice. Int Immunopharmacol. 2011; 11(9):1293-7. [DOI:10.1016/j.intimp.2011.04.011] [PMID] [DOI:10.1016/j.intimp.2011.04.011]
15. Jiménez-Medina E, Garcia-Lora A, Paco L, Algarra I, Collado A, Garrido F. A new extract of the plant Calendula officinalis produces a dual in vitro effect: Cytotoxic anti-tumor activity and lymphocyte activation. BMC Cancer. 2006; 6:119. [PMID] [PMCID] [DOI:10.1186/1471-2407-6-119]
16. Wang S, Huang S, Ye Q, Zeng X, Yu H, Qi D, Qiao S. Prevention of cyclophosphamide-induced immunosuppression in mice with the antimicrobial peptide sublancin. J Immunol Res. 2018; 2018:4353580. [DOI:10.1155/2018/4353580] [PMID] [PMCID] [DOI:10.1155/2018/4353580]
17. Esmaeili G, Van Laere K, Muylle H, Leus L. Artificial chromosome doubling in allotetraploid Calendula officinalis. Front Plant Sci. 2020; 11:622. [PMID] [PMCID] [DOI:10.3389/fpls.2020.00622]
18. Kontoghiorghe CN, Kolnagou A, Kontoghiorghes GJ. Phytochelators intended for clinical use in iron overload, other diseases of iron imbalance and free radical pathology. Molecules. 2015; 20(11):20841-72. [DOI:10.3390/molecules201119725] [PMID] [PMCID] [DOI:10.3390/molecules201119725]
19. Neboh EE, Ufelle SA. Myeloprotective activity of crude methanolic leaf extract of Cassia occidentalis in cyclophosphamide-induced bone marrow suppression in Wistar rats. Adv Biomed Res. 2015; 4:5. [DOI:10.4103/2277-9175.148285] [PMID] [PMCID] [DOI:10.4103/2277-9175.148285]
20. Fonseca YM, Catini CD, Vicentini FT, Nomizo A, Gerlach RF, Fonseca MJ. Protective effect of Calendula officinalis extract against UVB-induced oxidative stress in skin: Evaluation of reduced glutathione levels and matrix metalloproteinase secretion. J Ethnopharmacol. 2010; 127(3):596-601. [DOI:10.1016/j.jep.2009.12.019] [PMID] [DOI:10.1016/j.jep.2009.12.019]
21. Sen A. Prophylactic and therapeutic roles of oleanolic acid and its derivatives in several diseases. World J Clin Cases. 2020; 8(10):1767-92. [DOI:10.12998/wjcc.v8.i10.1767] [PMID] [PMCID] [DOI:10.12998/wjcc.v8.i10.1767]
22. Leopoldini M, Russo N, Toscano M. The molecular basis of working mechanism of natural polyphenolic antioxidants. Food Chem. 2011; 125(2):288-306. [DOI:10.1016/j.foodchem.2010.08.012] [DOI:10.1016/j.foodchem.2010.08.012]
23. Varish DA, Amrish K, Mansi V, Vipin KG. Gupta SK. Therapeutic Potential of Calendula officinalis. Pharm Pharmacol Int J. 2018;6(2):149-155. DOI: 10.15406/ppij.2018.06.00171 [DOI:10.15406/ppij.2018.06.00171]
24. Krummenauer ME, Lopes W, Garcia AWA, Schrank A, Gnoatto SCB, Kawano DF, et al. A highly active triterpene derivative capable of biofilm damage to control Cryptococcus spp. Biomolecules. 2019, 9(12):831. [PMID] [PMCID] [DOI:10.3390/biom9120831]
25. Küpeli Akkol E, Genç Y, Karpuz B, Sobarzo-Sánchez E, Capasso R. Coumarins and coumarin-related compounds in pharmacotherapy of cancer. Cancers (Basel). 2020, 12(7):1959. [PMID] [PMCID] [DOI:10.3390/cancers12071959]
26. Paya M, Halliwell B, Hoult R. Interactions of a series of coumarins with reactive oxygen species: Scavenging of superoxide, hypochlorous acid and hydroxyl radicals. Biochem Pharmacol. 1992; 44(2):205-14. [DOI:10.1016/0006-2952(92)90002-Z] [DOI:10.1016/0006-2952(92)90002-Z]
27. Stringlis IA, de Jonge R, Pieterse CMJ. The age of coumarins in plant-microbe interactions. Plant Cell Physiol. 2019; 60(7):1405-19. [PMID] [PMCID] [DOI:10.1093/pcp/pcz076]
28. Majnooni MB, Fakhri S, Smeriglio A, Trombetta D, Croley CR, Bhattacharyya P, et al. Antiangiogenic effects of coumarins against cancer: From chemistry to medicine. Molecules. 2019; 24(23):4278. [PMID] [PMCID] [DOI:10.3390/molecules24234278]

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