Volume 17 -                   Qom Univ Med Sci J 2023, 17 - : 462-471 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Rezaei S, Khoshsokhan-Mozaffar M, Kalhor N. Comparative Study of PRMT1 and MAPK14 Genes Expression in Healthy and Oligospermic Individuals. Qom Univ Med Sci J 2023; 17 : 2823.1
URL: http://journal.muq.ac.ir/article-1-3668-en.html
Comparative Study of PRMT1 and MAPK14 Genes Expression in Healthy and Oligospermic Individuals
Somaye Rezaei1 , Maryam Khoshsokhan-Mozaffar * 2, Naser Kalhor3
1- Department of Biology, Faculty of Basic Sciences, Qom Branch, Islamic Azad University, Qom, Iran.
2- Department of Biology, Faculty of Basic Sciences, Qom Branch, Islamic Azad University, Qom, Iran. , m.khoshm@gmail.com
3- Department of Mesanchymal Stem Cell, Academic Center for Education Culture and Research, Qom Branch, Qom, Iran.
Abstract:   (416 Views)
Background and Objectives: Male infertility is a complex disorder that affects a large portion of the male population. However, the causes of infertility are mainly unknown. Proper and complete implementation of spermatogenesis requires the expression of many genes. So, stopping or disrupting the expression of any of them may lead to blocking or upsetting the process of spermatogenesis. Identification of such genes and evaluation of their function provides valuable information about the role of these genes in adult spermatogenesis. The present study investigated the expression of PRMT1 and MAPK14 genes in healthy and oligospermic individuals.
Methods: Semen samples of normal sperm and oligospermia were collected according to WHO standards from individuals referring to Qom University, Jihad Infertility Treatment Center, after obtaining their consent. Semen samples were assessed regarding the expression of PRMT1 and MAPK14 genes in normal and oligospermic groups using real-time polymerase chain reaction (PCR). The data obtained were analyzed with the independent t test using PRISM software. The significance level was set at P<0.05.
Results: The study on cDNA from normal sperm and oligospermic individuals showed that the expression of the PRMT1 gene in oligospermic individuals increased 3.989 times compared to the control group, but the MAPK14 gene did not change significantly.
Conclusion: Increased expression of the PRMT1 gene in oligospermic individuals increases the process of apoptosis and ultimately reduces the number of healthy sperms.
Article number: 2823.1
Keywords: Oligospermia, PRMT1, Mitogen-activated protein kinase 14, Infertility, Men, Apoptosis
Full-Text [PDF 3418 kb]   (190 Downloads) |   |   Full-Text (HTML)  (197 Views)  
Type of Study: Original Article | Subject: ژنتیک
Received: 2023/02/4 | Accepted: 2023/07/9 | Published: 2023/08/1
References
1. Agarwal A, Said TM. Role of sperm chromatin abnormalities and DNA damage in male infertility. Hum Reprod Update. 2003; 9(4):331-45. [DOI:10.1093/humupd/dmg027] [PMID] [DOI:10.1093/humupd/dmg027]
2. McLachlan RI, Rajpert-De Meyts E, Hoei-Hansen CE, de Kretser DM, Skakkebaek NE. Histological evaluation of the human testis--approaches to optimizing the clinical value of the assessment: Mini review. Hum Reprod. 2007; 22(1):2-16.[DOI:10.1093/humrep/del279] [PMID] [DOI:10.1093/humrep/del279]
3. Li N, Saitou M, Atilla-Gokcumen GE. The role of p38 MAPK in triacylglycerol accumulation during apoptosis. Proteomics. 2019; 19(13):e1900160. [DOI:10.1002/pmic.201900160] [PMID] [DOI:10.1002/pmic.201900160]
4. Ríos-Arrabal S, Artacho-Cordón F, León J, Román-Marinetto E, Del Mar Salinas-Asensio M, Calvente I, et al. Involvement of free radicals in breast cancer. Springerplus. 2013; 2:404. [DOI:10.1186/2193-1801-2-404] [PMID] [DOI:10.1186/2193-1801-2-404]
5. Infantino S, Light A, O'Donnell K, Bryant V, Avery DT, Elliott M, et al. Arginine methylation catalyzed by PRMT1 is required for B cell activation and differentiation. Nat Commun. 2017; 8(1):891. [DOI:10.1038/s41467-017-01009-1] [PMID] [DOI:10.1038/s41467-017-01009-1]
6. Sermondade N, Faure C, Fezeu L, Lévy R, Czernichow S; Obesity-Fertility Collaborative Group. Obesity and increased risk for oligozoospermia and azoospermia. Arch Intern Med. 2012; 172(5):440-2. [DOI:10.1001/archinternmed.2011.1382] [PMID] [DOI:10.1001/archinternmed.2011.1382]
7. Vivanco I, Sawyers CL. The phosphatidylinositol 3-Kinase AKT pathway in human cancer. Nat Rev Cancer. 2002; 2(7):489-501. [DOI:10.1038/nrc839] [PMID] [DOI:10.1038/nrc839]
8. Richards JS, Sharma SC, Falender AE, Lo YH. Expression of FKHR, FKHRL1, and AFX genes in the rodent ovary: evidence for regulation by IGF-I, estrogen, and the gonadotropins. Mol Endocrinol. 2002; 16(3):580-99. [DOI:10.1210/mend.16.3.0806] [PMID] [DOI:10.1210/mend.16.3.0806]
9. Shi F, LaPolt PS. Relationship between FoxO1 protein levels and follicular development, atresia, and luteinization in the rat ovary. J Endocrinol. 2003; 179(2):195-203. [DOI:10.1677/joe.0.1790195] [PMID] [DOI:10.1677/joe.0.1790195]
10. Park Y, Maizels ET, Feiger ZJ, Alam H, Peters CA, Woodruff TK, et al. Induction of cyclin D2 in rat granulosa cells requires FSH-dependent relief from FOXO1 repression coupled with positive signals from Smad. J Biol Chem. 2005; 280(10):9135-48. [DOI:10.1074/jbc.M409486200] [PMID] [DOI:10.1074/jbc.M409486200]
11. Skarra DV, Arriola DJ, Benson CA, Thackray VG. Forkhead box O1 is a repressor of basal and GnRH-induced Fshb transcription in gonadotropes. Mol Endocrinol. 2013; 27(11):1825-39. [DOI:10.1210/me.2013-1185] [PMID] [DOI:10.1210/me.2013-1185]
12. Sinha D, Kalimutho M, Bowles J, Chan AL, Merriner DJ, Bain AL, et al. Cep55 overexpression causes male-specific sterility in mice by suppressing Foxo1 nuclear retention through sustained activation of PI3K/Akt signaling. FASEB J. 2018; 32(9):4984-99. [DOI:10.1096/fj.201701096RR] [PMID] [DOI:10.1096/fj.201701096RR]
13. Rossi BV, Abusief M, Missmer SA. Modifiable risk factors and infertility: What are the Connections? Am J Lifestyle Med. 2014; 10(4):220-231. [DOI:10.1177/1559827614558020] [PMID] [DOI:10.1177/1559827614558020]
14. World Health organization (WHO). WHO Laboratory Manual for the Examination of Human Semen and sperm-cervical mucus Interaction. Geneva: World Health organization; 1999. [Link]
15. Tahmasbpour E, Balasubramanian D, Agarwal A. A multi-faceted approach to understanding male infertility: Gene mutations, molecular defects and assisted reproductive techniques (ART). J Assist Reprod Genet 2014; 31(9):1115-37. [DOI:10.1007/s10815-014-0280-6] [PMID] [PMCID] [DOI:10.1007/s10815-014-0280-6]
16. Billig H, Chun SY, Eisenhauer K, Hsueh AJ. Gonadal cell apoptosis: Hormone-regulated cell demise. Hum Reprod Update. 1996; 2(2):103-17. [DOI:10.1093/humupd/2.2.103] [PMID] [DOI:10.1093/humupd/2.2.103]
17. Daneshmandpour Y, Bahramzadeh B, Yousefi M, Nouri M, sakhinia E. [The analysis of expression level of UBE2B and CX3CR1 in sperm cell of infertile patients (Persian)]. Paper presented at: The 6th International Biomedical Congress. 10 November 2022; Tabriz, Iran. [link]
18. Qin J, Wen B, Liang Y, Yu W, Li H. Histone modifications and their role in colorectal cancer (Review). Pathol Oncol Res. 2020; 26(4):2023-33. [DOI:10.1007/s12253-019-00663-8] [PMID] [PMCID] [DOI:10.1007/s12253-019-00663-8]
19. Jesenberger V, Jentsch S. Deadly encounter: Ubiquitin meets apoptosis. Nat Rev Mol Cell Biol. 2002; 3(2):112-21. [DOI:10.1038/nrm731] [PMID] [DOI:10.1038/nrm731]
20. Lippert TH, Seeger H, Schieferstein G, Voelter W. Immunoreactive ubiquitin in human seminal plasma. J Androl. 1993; 14(2):130-1. [DOI:10.1002/j.1939-4640.1993.tb01666.x] [PMID] [DOI:10.1002/j.1939-4640.1993.tb01666.x]
21. Sinha Hikim AP, Swerdloff RS. Hormonal and genetic control of germ cell apoptosis in the testis. Rev Reprod. 1999; 4(1):38-47. [DOI:10.1530/ror.0.0040038] [PMID] [DOI:10.1530/ror.0.0040038]
Add your comments about this article : Your username or Email:
CAPTCHA
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2025 CC BY-NC 4.0 | Qom University of Medical Sciences Journal

Designed & Developed by : Yektaweb