Ethics code: IR.IAU.VARAMIN.REC.1399.002
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Shahriary S, Tafvizi F, Parvin K, Shaabanzadeh M. Cytotoxicity Effect of Artemisia deserti Aqueous Leaf Extract on Cis-platin Resistant Ovarian Cancer Cell Line (A2780-CP). Qom Univ Med Sci J 2024; 18 : 913.3
URL: http://journal.muq.ac.ir/article-1-3768-en.html
URL: http://journal.muq.ac.ir/article-1-3768-en.html
1- , farzanehtafvizi54@gmail.com
Abstract: (338 Views)
Background and Objectives: Cancer is the second leading cause of human death in the world while chemotherapy is one of the main therapeutic approaches to manage this disease. Considering numerous side effects and different cases which showed up resistance features, many studies looking for some alternatives to control this issue. One of the proposed substitutes is herbal medicine. This choice introduces many active ingredients such as terpenoids and flavonoids which can interrupt cell proliferation. In this study the effect of aqueous leaf extract of Artemisia deserti was examined on cisplatin resistant ovarian cancer cell line A2780-CP.
Methods: First, Artemisia deserti extract was prepared through boiling the dried leaf powder. MTT assay was performed to calculate the IC50 concentration level of the prepared extract (112.62 µg/mL) in order to treat the under-study cell line A2780- CP. Generation of intercellular ROS has been measured. Induction of apoptosis and cell cycle arrest was analyzed by flow cytometry assay via Annexin V/PI and fluorescence staining. Moreover, qRT-PCR was performed to measure the expression level of genes such as BCL2, BAX, CASP3, CASP9, P21, CCNB1, CCND1, and TFRC.
Results: The overall results revealed a dose dependent cytotoxic effect of Artemisia deserti leaf extract on cisplatin resistant ovarian cancer cell line A2780-CP. Generation of intracellular ROS level, along with significant changes in expression level of candidate cell cycle and apoptotic genes was observed in this study. It emphasized that the potency of extract solution to induce apoptosis was quite significant. The results also indicated that the prepared aqueous leaf extract has negligible cytotoxicity effect on normal healthy Human Foreskin Fibroblasts cell line (HFF).
Conclusion: Finally, the findings of this study propose Artemisia deserti aqueous leaf extract as a good candidate for further in-vitro studies on cisplatin resistant cancer treatment approaches.
Methods: First, Artemisia deserti extract was prepared through boiling the dried leaf powder. MTT assay was performed to calculate the IC50 concentration level of the prepared extract (112.62 µg/mL) in order to treat the under-study cell line A2780- CP. Generation of intercellular ROS has been measured. Induction of apoptosis and cell cycle arrest was analyzed by flow cytometry assay via Annexin V/PI and fluorescence staining. Moreover, qRT-PCR was performed to measure the expression level of genes such as BCL2, BAX, CASP3, CASP9, P21, CCNB1, CCND1, and TFRC.
Results: The overall results revealed a dose dependent cytotoxic effect of Artemisia deserti leaf extract on cisplatin resistant ovarian cancer cell line A2780-CP. Generation of intracellular ROS level, along with significant changes in expression level of candidate cell cycle and apoptotic genes was observed in this study. It emphasized that the potency of extract solution to induce apoptosis was quite significant. The results also indicated that the prepared aqueous leaf extract has negligible cytotoxicity effect on normal healthy Human Foreskin Fibroblasts cell line (HFF).
Conclusion: Finally, the findings of this study propose Artemisia deserti aqueous leaf extract as a good candidate for further in-vitro studies on cisplatin resistant cancer treatment approaches.
Article number: 913.3
Type of Study: Original Article |
Subject:
سلولی و مولکولی
Received: 2023/07/3 | Accepted: 2023/08/20 | Published: 2024/04/29
Received: 2023/07/3 | Accepted: 2023/08/20 | Published: 2024/04/29
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Owned by Qom University of Medical Sciences
Published by Qom University of Medical Sciences Press
eISSN: 2008-1375
pISSN: 1735-7799
Email: journal[at]muq.ac.ir
j.mua.ac[at]gmail.com
